Sentence examples for using the amplified probes from inspiring English sources

The obtained cosmid clones were diluted 104-106 fold to give around 5×103 colonies per LB plate, and screened the bafilomycin biosynthetic gene cluster using the amplified probes labeled with α-[32P]- dCTP.

The disruption of pta and ack genes was further confirmed by southern blotting using the amplified 486 bp probe.

A cDNA encoding this protein was obtained by screening a saccular cDNA library using the amplified cDNA fragment as a probe.

In the present work, we designed a unique enzyme-aided multiple amplification strategy for sensitive electrochemiluminescence (ECL) detection of DNA by using the amplified gold nanoparticles (GNPS -polyamidoamine (PAMAM)-CdSe quantum dots (QDs) siGNPS -polyamidoamine

using the amplified miRNA samples.

The RNA was also subject to poly-A+ selection (20 mg of oligo-dT per 500 µg of total RNA, Ambion, Austin, TX, USA) and northern blotting using PCR amplified probes for each gene in the dcr interval.

The amplified probe was radiolabeled and used to screen a B. ovis cDNA library.

Detection of the amplified probe was done with the Duolink II Detection Reagents Red Kit.

Labeled probe was produced from an aliquot of the amplified probe with Klenow fragment and 32P-labeled nucleotide.

In addition, different methylation patterns were observed in seven tissues from pig, and almost all of the methylation patterns detected by F-MSAP could be confirmed by Southern analysis using the isolated amplified fragments as probes.

In the present study, amplified products by PCR were analysed by Southern blot hybridization using the ECL-labelled probes obtained from cloned GP5+/GP6+ amplified products of HPV-positive control DNAs.