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Denoised 3-D reconstructions were manually segmented using the Amira software package (Mercury Computer System, Inc). on a Wacom Cintiq 21UX display.
The analysis of body fat mass was performed using the Amira software (Visage Imaging GmbH).
Deconvolution was performed using Richardson-Lucy iterations and movies were made using the Amira software (FEI).
Using the AMIRA software package the 2D tumour sections will be stacked to render a 3D reconstruction of the histopathology.
Using the AMIRA software package the outlined MRI images will be stacked to render a 3D reconstruction of the kidney and the IRE ablation zone within.
Measurements of coat lattices and visualization (Gaussian filtering of volumes, snapshots of slices and movies through volume slices) were performed using the AMIRA software package (VSG, Merignac, France).
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Fig. 4 Creation of 3D reconstructions using the Amira 4.1.1® software (Mercury Computer System, Inc., Chelmsford, MA, USA).
The mapping of the 3D gene expression data and the GFP fate maps to the reference models was performed using the Amira 4.1 software from Mercury Computer Systems.
Volume-rendered 3-D images were obtained from the vertically stacked 2-D slices using the Amira 5.2 software (Visage Imaging, San Diego, CA, USA).
To obtain 3-D coordinates of the PC dendrites, reconstructed image stacks of PC were segmented and skeletonized using the Amira 5.2 software.
The 3D reconstitution was done using the Amira 3D v4.1.1 Imaging program.
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