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Reconstructions were segmented using the Amira module "LabelField" to maximize vessel visibility and aid in image interpretation.
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Surface renderings were created using the Amira "Isosurface" module with a threshold chosen just above the noise floor.
The cropping was done manually using the Amira VolumeEdit module, the Hoxd13 gene expression pattern was used as a guide and 2 different methods were used.
These union domains were calculated using the Amira arithmetic module from every domain from all grafting experiments for a specific digit at a specific stage.
The unique domains are these stage specific digit union domains masked for the union domains of the other digits, the unique domains were also calculated using the Amira arithmetic module.
Volume renderings were created using the Amira "Voltex" module, with the low threshold being chosen just above the noise floor and the high threshold chosen to maximize vessel visibility.
Fig. 4 Creation of 3D reconstructions using the Amira 4.1.1® software (Mercury Computer System, Inc., Chelmsford, MA, USA).
The 3D reconstitution was done using the Amira 3D v4.1.1 Imaging program.
The mapping of the 3D gene expression data and the GFP fate maps to the reference models was performed using the Amira 4.1 software from Mercury Computer Systems.
Using the Amira Voltex feature, we determined that the threshold at which we no longer observed background signal was 50 on the 8 bits greyscale.
Denoised 3-D reconstructions were manually segmented using the Amira software package (Mercury Computer System, Inc). on a Wacom Cintiq 21UX display.
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