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Herein, we evaluated the mutagenicity of a series of 7-azabicyclo[2.2.1]heptane 7-azabicyclo[2.2.1]heptane monocyclic nitrosamines by using the ands assay.
The measured mutagenicity, using the Ames assay test with TA98 and YG1024 strains of Salmonella typhimurium indicate a dose response for the nitroarene sensitive YG1024 strain for B100 and PPO (fold induction: 1.6).
Besides, we envisaged the study of the antimutagenic activity of the same extracts toward four different mutagens, having diverse chemical structures and mode of actions, by using the Ames assay.
Also using the comet assay, Liviac et al. (2010) found that pool water was more genotoxic than the source tap water; a similar analysis using the Ames assay would help in characterizing the relative mutagenicity of drinking versus pool water.
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With the increasing of the database templates, the proposed method will be much more effective than using the AME-SAD approach.
The resolution level of MRMEH is from 1 to 7. In our experiment, the classification rate is 96.39% using the AME-SAD and 1-NN rule.
MEME-ChIP also performs motif enrichment analysis using the AME algorithm, which can detect very low levels of enrichment of binding sites for TFs with known DNA-binding motifs.
Fibroblasts invasion assay was measured using the transwell assay as described previously20.
Protein concentration was determined using the Bradford assay.
Cell proliferation was determined using the EdU assay.
Luciferase assays with cell lysates were performed within 3 days of transfection using the Luciferase Assay System (Promega).
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