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A more ambitious strategy involves aligning reads to a reference genome and using the alignments to infer the transcript structures.
A more ambitious method involves aligning reads to a reference genome and using the alignments to infer the transcript structures, possibly with the aid of a curated transcript database.
The time to align the homologs to the query might be reduced by using the alignments implied by the families as a starting point to search for local alignments, instead of using BLAST to realign the homologs to the query.
All primers were checked using the alignments of sequences, and subsequently with the basic local alignment search tool (BLAST; http://www.ncbi.nlm.nih.gov/BLAST/).nih.gov/BLAST/
Second, using the alignments and triphone tree structures of the clean model, reverberated acoustic models were trained on the MC dataset according to the ML criterion.
As such, overlay of two conformers, A and B, to a reference conformer, R, may generate an excellent approximation to the ideal alignment of conformers A and B by simply (re)using the alignments AR and BR.
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– Find small indels and SNVs in aligned regions (using the alignment information produced by LAGAN).
We aligned protein sequences using the alignment utility of MEGA 5.0 [ 36].
Patterns may be aligned to existing alignment marks without exposing the writing area by using the alignment program AL.
Subsequently residues were translated to the aminergic GPCRs counterpart using the alignment from GPCRdb [48].
In Figure 6, additional results are given using the alignment method described in [15].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com