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Sequences were aligned using the alignment tool of the ARB package (http://www.arb-home.de/) and Bioedit software (http://www.mbio.ncsu.edu/BioEdit/bioedit).
As a control, the reads were first aligned using the alignment tool provided by Applied Biosystems (mapreads) against reference sequences representing the fully bisulfite converted Watson and Crick strands and the unconverted Watson strands of A.thaliana and phage lambda.
Different azo-reductase protein sequences from various microorganisms were aligned against those of B1 P. aeruginosa using the alignment tool of CLC Main Workbench 5 (CLC Bio, Aarhus, Denmark).
The sequence obtained was compared with sequences available in EMBOSS database via http://www.ebi.ac.uk/emboss/align/ using the alignment tool.
A multiple sequence alignment was constructed using the alignment tool in Geneious Pro version 6.1.6 (Drummond et al., 2011) before performing manual checks and minor adjustments.
We mapped the pig genome onto the human genome using the alignment tool (LAST) and found the syntenic region; the pig chromosomes are rearranged in human chromosome scale.
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Students also reported strong increases in their confidence in using the alignment tools, Geneious and BLAST.
In this step, VERSE uses the alignment tool Bowtie 2 [ 35] to map raw sequencing reads to the reference genome of the host species under study.
As genomes may undergo large-scale changes during evolution, global alignments using the Mauve alignment tool [ 17] were performed by pairwise comparison of the seven C. concisus strains with the reference BAA-1457 strain.
The trimmed sequences from each barcode bin were aligned using the NAST alignment tool (http://greengenes.lbl.gov) [32].
Then, the regions between conserved motifs were multiply aligned using the Clustal alignment tool found in the application MEGA [ 53].
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