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Using the alignment to the WS190 reference genome, small insertions can be characterized by clusters of PETs that are shorter than the average size, whereas deletions in the sample can be detected by PETs that are longer.
Secondly, searches for the site of phosphorylation were undertaken by constructing alignments of the 10 amino acid residues surrounding the conserved histidine from all predicted HKs in each replicon, and using the alignment to create a profile.
Subsequently, this information was collected and, by using the alignment to track the same position on different kinase-substrate structures, the minimum distance for each alignment position was obtained.
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A more ambitious strategy involves aligning reads to a reference genome and using the alignments to infer the transcript structures.
A more ambitious method involves aligning reads to a reference genome and using the alignments to infer the transcript structures, possibly with the aid of a curated transcript database.
Step 10: Finally, using the alignments to the reference genome, we compute precisely where the de novo mutations occur.
Placements were checked for accuracy using the alignments to the sequenced BACs, as the location of the MSLL inserts on the FPC map should correspond to the location of the BAC that it aligned to.
After finding the alignment parameters for these resolutions, we use the alignment to register the facial images using an interocular distance of 50 pixels.
We then used the alignment to investigate whether differences between genomes assembled with Illumina and 454 datasets were likely to be due to SNPs or sequencing error and in what regions of the genome the SNPs occurred.
In addition, we used the alignment to establish the Neighbor-Joining (NJ) phylogenic tree to study the relationship between PL10, DDX3X, and DDX3Y by MEGA4 [ 55] with the Maximum Composition Likelihood approach and 1000 bootstrap replicates (Fig. 1).
Thus using the alignment score to report the best hit is not as good as using the alignment ratio.
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