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To study the evolutionary dynamics and turnover rates of cellulase genes, we reconstructed the phylogenetic relationship of the 12 Pristionchus ESTs encoding CBM49 domains by the maximum likelihood criterion using the alignment shown in additional file 1.The resulting phylogram revealed two important findings.
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We used the alignment shown in Figure 1 to construct a phylogeny of bacteriorhodopsins (see Additional file 1 for the full alignment).
The 30 conformations of the C-terminus of OxB were homology modeled on the basis of the 30 conformations of OxA using the alignment as shown in Figure 2A with MOE (version 2010.10, Chemical Computing Group).
The alignment shown was used for generation of the network presented in figure 5. M. mulatta and N. leucogenys represent the source sequence of the LAVA 3' part in Hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3 ) intron 2. Figure S5.
The validated targets used to create the alignment showed a greater degree of heterogeneity that those in other alignments.
Up to date, shifts in base composition of D-loop Numts have not been studied, as cases of noncoding Numts have rarely been reported [ 4, 9, 10, 16- 18]. 3. Phylogenetic analyses of these results using the alignment in Figure 2 are shown in Figure 3. Sequences CH.1.4 and CB.1.1 were not the most divergent, but they were the only ones sharing a TCCCC insertion at positions 198 202.
A result image of adjusted alignment is demonstrated to show the reduced discrepancy by using the alignment measurement method.
The alignment showed negligible errors.
The (R)- or (S -configurations of the tetrahedral intermediate in the different variant S -configurationsofpared theough alignmentetrahedralrrespondintermediateatinns in the wildifferentzyme by using MUSTANG.[ 38] The alignments showed that the mutations induce movariantof the phenyl groupstructuresult of steric hindrance.
These latter strains are representatives of the most widespread clonal complexes 1 and 2. Gene order comparison using MAUVE alignment showed multiple changes and 19 regions with high homology named Local Colinear Blocks (LCBs), which encompassed an average of 81.73% of each genome (fig. 2).
The score of each left out target along with the number of regions with a score equal to or greater than this value in the scan using the full alignment are shown in Table 3. "target" gives validated target sequence accession/start-end; "miRNA" gives miRNA targeting that region; "⩾ LOO score" gives mean number of regions scoring equal to or greater than the left out sequence.
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