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In order to investigate the relationship of VerFtsZ to FtsZ of other bacteria, we performed phylogenetic analysis using the alignment in Fig 1 which consists of a set of reference sequences of FtsZ of bacteria of different phyla.
Up to date, shifts in base composition of D-loop Numts have not been studied, as cases of noncoding Numts have rarely been reported [ 4, 9, 10, 16- 18]. 3. Phylogenetic analyses of these results using the alignment in Figure 2 are shown in Figure 3. Sequences CH.1.4 and CB.1.1 were not the most divergent, but they were the only ones sharing a TCCCC insertion at positions 198 202.
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The program uses the alignment in question and returns files with informative and unambiguous sites as well as a log file containing the PHI p-values.
A multiple sequence alignment was constructed using the alignment tool in Geneious Pro version 6.1.6 (Drummond et al., 2011) before performing manual checks and minor adjustments.
The three-dimensional structure of target protein was predicted by using the alignment file in MODELLER software [ 16] whereby predicted structure was returned.
In Figure 6, additional results are given using the alignment method described in [15].
The core functionality of AlignGraph is the extension of the contigs by reassembling them using the alignment results obtained in the previous step.
Many of these cases could be discerned in advance by using the alignment quality measures presented in Section 2.2.4.
Sequence divergence was calculated using the alignment region with sequence in all species compared.
Students also reported strong increases in their confidence in using the alignment tools, Geneious and BLAST.
We used the alignment shown in Figure 1 to construct a phylogeny of bacteriorhodopsins (see Additional file 1 for the full alignment).
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