Using the aforementioned procedure, the effects of a concentration series of HEPES was tested.
L4 were cultured from day 14 L3 isolated using the aforementioned procedure.
Polyplex solutions were prepared at an N/ P ratio of 5 using the aforementioned procedure for transgene expression and diluted with Opti-MEM prior to transfection.
To assess the influence of P presence on arsenate sorption, experiments with solutions of equimolar As V): P concentrations were carried out, using the aforementioned procedure and concentrations used for the As (V) sorption experiments.
Five hundred microliters of plasma was centrifuged at 300 g for 4 min, filtered through a 0.22-μm pore-sized filter, and then purified by SEC using the aforementioned procedure.
Fixed tissues on slides or freshly prepared, floating meninges were stained using the aforementioned procedure but replacing the ABC complex with 30 min to 1 h incubation in Fluorescein Avidin DCS, or Texas Red Avidin D (Vector Laboratories).
Four sets of GO annotation were produced using the aforementioned procedure: all expressed genes in E18, all expressed genes in P7, genes with intronic TAR(s) in E18 and genes with intronic TAR(s) in P7.
More specifically, we first impaired a state-of-the-art SIFT-based copy-move forgery detector (briefly CMFD [3] by means of the CLBA attack[13]; then, we hid potential traces of keypoint removal using the aforementioned injection procedure.
Three distinct dietary patterns, labelled 'processed meat pattern', 'prudent vegetable pattern' and 'high-sugar pattern', were extracted using the aforementioned factor analysis procedure.
It was calculated using the aforementioned genetic data and procedures outlined in literature published by several direct-to-consumer genetic testing companies (see Appendix D for an example) [ 52- 54].
