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Microarray data sets were analyzed using the Affymetrix tiling array software (TAS).
RNA mapping experiments using the Affymetrix tiling array were performed as previously described [38].
Triplicate samples were combined and the Foxo precipitated samples were compared to mock precipitated samples using using the Affymetrix Tiling analysis (TAS) software.
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To identify the H3K9Ac enriched sites, we performed ChIP followed by tiling array analysis (ChIP on chip) using the Affymetrix mouse tiling 1.0 array, which spans the entire mouse genome at a 30 bp resolution with 47 million 25-mer probes (excluding repetitive sequence elements).
Using the Affymetrix Arabidopsis Tiling 1.0 Array, consecutive stretches of SFPs were observed that may be caused by an accumulation of many sequence alterations in a certain region resulting in highly deviant non-Col-0 alleles or may be due to deletions.
By using the Affymetrix Arabidopsis Tiling 1.0 Array and analysing 54 accessions, we substantially increased the coverage of the A. thaliana population analysed by deep genotyping and phenotyping at the cost of not covering29% of the genome and not knowing the exact nature of the underlying polymorphism.
The tiling array analysis was carried out using the Affymetrix C. elegans Tiling 1.0R Array containing 25-mer perfect match (PM) and mismatch (MM) probes tiled over the C. elegans genome.
Transcript abundance of three biological replicates of three-week-old leaves and stems, and seven day old roots was measured using the Affymetrix B. distachyon BradiAR1b520742 whole genome tiling array.
Samples were hybridized to tiling arrays and scanned at the BioMedical Genomics Center at the University of Minnesota using the Affymetrix Fluidics Station 400.
Biological duplicates were analysed using the Affymetrix GeneChip Yeast Gene 1.0 ST Array and the GeneChip® 3' IVT Express protocol (Affymetrix, Santa Clara, CA, USA).
The additional second distribution covers those transcripts that would have gone undetected using the Affymetrix technology.
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