Sentence examples for using the affymetrix protocol from inspiring English sources

Biotinylated target RNA was prepared from 5 µg of total RNA using the Affymetrix protocol.

cRNA synthesis was carried out using the Affymetrix protocol with minor modifications as previously described [40].

For subsequent fragmentation using the Affymetrix protocol the original amplification protocol was modified by adding 2.3 µl of 10 mM dUTPs in the PCR master mix (total volume per reaction: 61 µl).

Two µg of total testicular RNA was isolated from three independent individuals of each species and used to prepare biotinylated single-stranded target using the Affymetrix protocol (Whole Transcript (WT) Double-Stranded Target Assay Manual, 702179 Rev. 3, Affymetrix) with a slight modification: the synthesis of second-strand cDNA was omitted in the final step.

cRNA synthesis was carried out using the Affymetrix protocol with minor modifications as described [ 26].

Biotinylated target RNA was prepared from 15 μg of total RNA using the Affymetrix protocol.

Approximately 2.5 µg of RNA were labeled using the Affymetrix labeling protocol (Affymetrix, Santa Clara, CA).

RNAs hybridized to Affymetrix chicken GeneChips using the Affymetrix standard protocol (Affymetrix UK Ltd ,High Wycombe, UK).

RNA was isolated according to standard procedures and hybridized to Affymetrix chicken GeneChips using the Affymetrix standard protocol (Affymetrix UK Ltd ,High Wycombe, UK).

Approximately 2.5 μg of RNA were labeled using the Affymetrix labeling protocol (Affymetrix, Santa Clara, CA, USA), with cRNA samples hybridized to Affymetrix Canine_2 gene expression chips as described [ 40].

Detailed protocols for sample preparation using the Affymetrix labeling protocols can be found at [ 44].