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These additional regions of CNNLOH identified using the Affymetrix data may to a large extent be false positives due to the larger experimental noise.
The Agilent threshold was set so that the fraction of I-SPY 1 samples in the Agilent data set predicted to be sensitive was the same as that predicted to be sensitive using the Affymetrix data (see Supplementary Materials).
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We used the Affymetrix data from TCGA [ 9] (n = 549; genes = 13,104) as a discovery data set.
In addition, evaluated genes were chosen by a data-driven approach, using the Affymetrix® data as an exploratory data set.
The gene expression analysis was performed using GeneSpring GX 7.3 (Agilent) and data were normalized using the Affymetrix FE data normalization procedure recommended for two-colour Agilent microarrays.
We also examined the Spearman correlation between p-values calculated using the Affymetrix array data with each of the six testing methods and qRT-PCR data for the 47 qRT-PCR assayed genes.
The hybridization data were analyzed using Chromosome Analysis Suite (ChAS) software, and the CNV loci results were calculated using the Affymetrix reference data as the control.
Again using the Affymetrix GeneChip data from the MDA-MB-435/β4 MDA-MB-435/β4 MDA-MB-435/β4e in the expression of genes surrounding the miR-100 cellser despite doweregulation of miR-100 in this system (detected showno.
Using the Affymetrix expression data, four gene sets were created: genes up- or down-regulated > 2.0 fold by iWT with R5020, and genes up- or down-regulated > 2.0 fold by iKR with R5020.
TEDS children were genotyped at the Affymetrix service laboratory using the Affymetrix GeneChip 6.0 and data were cleaned as previously described (Davis et al. 2014).
To compare our microarray data using the Affymetrix chip with the data using the non-commercial microarray containing 50mer oligonucleotides [15], we searched in Xenbase [46] with the 50mer oligonucleotide sequence the corresponding Affymetrix probeset numbers for the transcripts of the seven categories with an altered adenylation behavior at maturation or upon fertilization.
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