The additional modification of Arn1, thus, needs to be determined.
In order to show the effect of the extra steps added to the commercial RNA extraction protocol we also performed the same experiment on RNA extracted using the kit without modifications (Additional file 1: Figure S5).
Additionally, various efforts are also being undertaken to improve the sensitivity of luminescent strains by using different or additional modifications.
This leads to the conclusion that the theoretical maximum rate of lactate production, equal on a carbon basis to the maximum rate of carbon fixation (the dashed line in Figure 4, which can be calculated from the reported maximum growth rate [ 42]), can only be reached by using different and/or additional modifications, genetic or physiological.
These tested and reference mixtures were used without additional modifications in fluorescence and light extinction measurements.
On the other hand, after 100-fold dilution, the responses to cysteine and dopamine became negligible, and response to all interferents in total was only about 20% of the response to hydrogen peroxide, which means that in some cases, the sensor can be used even without additional modifications.
After agents reviewed the recommendations, Redfin was able to use the agents' modifications as additional training data, and the click-through rate on recommended houses rose significantly.
Primers were designed based on criteria stated by Wojdacz et al. [ 9] with some additional modifications using the Methyl Primer Express v1.0 Software Appliedd Biosystem, USA).
qRT-PCR was performed using the primers listed in Additional file 1: Table S1 as previously described [ 28], with a minor modification of the PCR program.
DNA extracted using Method S with no additional modification was not included in stage 1.
