Sentence examples for using the absolute quantification from inspiring English sources

Crossing points were calculated by the LightCycler®Software 4.0 (Roche Diagnostics) using the absolute quantification fit points method.

Cp values were converted to copy numbers using the absolute quantification method based on Cp values for the genomic DNA standards.

The DNA integrity was checked and quantified using the absolute quantification in real-time PCR as suggested by Applied Biosystems (Foster City, CA, USA).

Calculation of the gene copy number was carried out using the absolute quantification method.

Crossing points (Cp) were calculated by the LightCycler®Software 4.0 (Roche Diagnostics GmbH) using the absolute quantification fit points' method.

The levels of all examined transcripts were determined using the absolute quantification method in seven mouse tissues (Fig. 5, Table 1).

If the anatomical information is used, the absolute quantification of all the segments improves when compared to all other algorithms.

We purposely used the absolute quantification or the standard curve method because only this procedure firmly generates several key indices.

For expression measurements, we used the absolute quantification analysis from the LightCycler Software 4.0 package (Roche), and calculated expression levels relative to wild type values.

For expression measurements, we used the absolute quantification analysis from the LightCycler Software 4.0 package (Roche), and calculated expression levels relative to values of a reference sample.

Since metabolite concentrations spanned more than five orders of magnitude, the splitless injection method used in the study permitted their identification, but it could not be used for the absolute quantification of highly abundant metabolites (e.g., sugars).