Sentence examples for using the above primers from inspiring English sources

Optimization of RT-LAMP reaction at various conditions was then carried out by using the above primers.

The entire gene for PaXynG was obtained by PCR amplification using the above primers and genomic DNA of P. antarctica GB-4 0) GB-4 0plase.

To obtain the whole cDNA sequence of AST-C (GenBank accession no. AB330418), RACE was performed by using the above primers and the SMART RACE cDNA Amplification Kit.

Detection of Pdro mRNA expression in human tissues (Total RNAs from BD Biosciences) was done by RT-PCR using the above primers.

The kinase-dead ppk1 carrying mutations of two histidine residues (required for autophosphorylation) to alanine [17] [ppk1-KD] wamplifiedied by PCR from a suitable construct essentially as described by Sureka et al. [17] using the above primers and integrated into M. smegmatis mc2155 as described above.

PCR was conducted using the above primers specific to castor FAD2 gene and 0.5 μL cDNA from the RT reaction.

PCR was carried out using the above primer sets with an initial 1 min denaturation at 94°C, followed by 30 cycles at 94°C for 15 sec, at 55°C for 30 sec, and at 72°C for 1 min. This was followed by a final extension of 5 min at 72°C.

Using the above primer selection criteria, we generated an average of 1555.8 +/- 1249.4 primer pair candidates per SNP.

PCR amplification of yeast DNA using the above primer pairs showed good quality products of 98.8% of experiments.

The tanA gene (NCBI accession number AB244239) along with flanking restriction sites was PCR amplified using the above mentioned primers along with genomic DNA isolated from S. lugdunensis as template.

Similar results were obtained by probeset 204748_at and by qRT-PCR using the above mentioned primers.