In this article, we apply the fixed point theorem of mixed monotone operators to get the existence and uniqueness of the iterative solutions for singular fractional differential equations (1.1) without using the above condition (b).
Hereinafter, the set of points situated on the actual contour of the tomato computed using the above condition is represented as (mathbf {P}^{i}= left {{P_{h}^{i}},h=1,2,ldots {n_{P}^{i}}right }), where ({n_{P}^{i}}leq n_{P_{v}}^{i}).
The activation energy was calculated and found to be 6.38 k.Cal.Mol -1. Figure 10 Semilogarithmic plot of SER (20.0 μg/mL) versus different heating times with 0.1% (v/v) H 2 O 2 then with 0.2% NBD-Cl using the above conditions.
For making a standard curve of flavonoids, seven standard working solutions with 1, 2, 5, 20, 50 and 80, and 100 ng/mL were made by diluting from high concentration stock solutions, and analyzed by HPLC DAD MSn using the above conditions, sequentially.
In the doubly targeted allele in clones C1-C3, PC and PD are 4 kb apart and no PCR product is generated using the above conditions.
The supernatant was then removed, placed in a separate 15 ml falcon tube and centrifuged again using the above conditions.
The seminiferous tubules were then incubated in 50 ml EKRB containing 0.5 mg trypsin/ml and l μg DNase/ml for 15 min using the above conditions.
The HPTLC analysis was carried out using the above conditions and the developed plates were visualized under UV light and densitometric scanning was performed to obtain the Rf values and corresponding concentration of the spots (AU) [ 14].
After both forward and reverse N primers and the Platinum Pfx DNA polymerase (Invitrogen) were added, the DNA synthesis was completed by using the above conditions for the N primers in a one-step RT-PCR.
All three fragments were amplified in 40 μL volumes using the above conditions with the exception of magnesium chloride concentration, which was lowered to 2 mM, and annealing temperature, which was raised to 65°C, to increase specificity.
AChE was encapsulated using the above conditions with ten freeze-thaw cycles followed by ten extrusions through a polycarbonate filter usually sold for the liposome extruder (Fig. 4, line 1).
