Sentence examples for using the abc system from inspiring English sources

After blocking endogenous peroxidase activity with 3% H2O2 and 0.2 M sodium azide in methanol, primary antibodies were detected using the ABC system (DAKO, Hamburg, Germany) with 3,3'-diaminobenzidine tetrahydrochloride as substrate.

After several washes in PBS, antibody complex was localized using the ABC system (Vectastain ABC Elite kit cat #PK6101, Vector Laboratories) followed by 3,3'-diaminobenzidine reaction with nickel enhancement.

For immunohistochemistry, a biotinylated anti-rabbit antibody was used at 1 200 and developed using the ABC-DAB system (Vector Laboratories).

Tbx18 and Hcn4 stainings were enhanced using the ABC (Avidin Biotin-Complex) reAvidin Biotin-Complexoreagents (VECTASTAIN Elite Peroxidase ABC-Kit) and the TSA (Tyramide Signal Amplifromtion) system (NEL741, Perkin Elmer) according to the manufacturers' instructions.

Immunoreactivity was detected using the ABC-peroxide based system (DAKO) following the manufacturer's protocol.

Floating sections were processed for IHC using the VECTASTAIN ABC system (Vector Laboratories, Burlingame, California, USA) and diaminobenzidine (Sigma-Aldrich) in combination with polyclonal rabbit anti-Cre (a generous gift from G. Schütz, DKFZ, 1 3000) and polyclonal rabbit anti-GFP (Invitrogen, Darmstadt, Germany 1 1000) antibodies.

The secondary reaction for the GFP was then visualized using the ABC-DAB and for visualizing the NeuN, we used ABC-AP Vector Red SK-51000, Vector Labs, Burlingame, CA).

Immunohistochemistry was performed using the ABC-Kit from Dakocytomation.

HEPACAM1 expression was immunohistochemically detected using the ABC-method.

Appropriate biotinylated secondary antibodies were used for detection using the ABC-method (Vector Laboratories).

Immunostaining was preformed using the ABC-technique (Vector Laboratories, Elite Standard Kit. Cat. PK-6100, Burlingame, CA, USA).