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After incubation with the biotinylated-secondary antibody (Vector), labeling was detected using the ABC staining kit (Vector).
Immunohistochemical detection was performed according to the avidin biotin complex method using the ABC staining kit (Santa Cruz Biotech INC., CA, USA).
The specimens were then incubated with the secondary antibody, anti-mouse IgG, for 1 h at 37°C and stained by the avidin-biotin peroxidase complex (ABC) method using the ABC staining system (Santa Cruz Biotech, Santa Cruz, CA, USA).
Next, the targeted protein was detected using secondary antibodies followed by detection using the ABC staining system (Santa Cruz, Santa Cruz, CA, USA), and the sections were counterstained with hematoxylin.
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The tumour sections were subsequently washed with PBS and stained using the ABC-AP kit and the Vector Red alkaline phosphatase substrate solution.
The secondary reaction for the GFP was then visualized using the ABC-DAB and for visualizing the NeuN, we used ABC-AP Vector Red SK-51000, Vector Labs, Burlingame, CA).
Immunohistochemistry was performed using the ABC-Kit from Dakocytomation.
HEPACAM1 expression was immunohistochemically detected using the ABC-method.
Appropriate biotinylated secondary antibodies were used for detection using the ABC-method (Vector Laboratories).
Immunoreactivity was detected using the ABC-peroxide based system (DAKO) following the manufacturer's protocol.
Immunostaining was preformed using the ABC-technique (Vector Laboratories, Elite Standard Kit. Cat. PK-6100, Burlingame, CA, USA).
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