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Immunoreactivities were visualized using the ABC method (Vector).
Immunohistochemical staining was performed using the ABC method (Vector Laboratories, Burlingame, CA).
For PV staining, sections were first incubated in a solution of a PV-antibody (SIGMA-Aldrich, dilution of 1∶4000, 0.1% Triton) for 48 hours, then in a solution of a secondary antibody (biotinylated anti-mouse, SIGMA Aldrich, 1∶200) for two hours and visualized using the ABC method with α-chloronaphtol as the chromogen.
The indirect costs which were calculated using the ABC method are shown in Table 4.
The sections were processed for immunohistochemistry using the ABC method (Yamanaka et al., 2004).
A high feasibility rate was reported when using the ABC method [ 10, 20].
Similar(30)
HEPACAM1 expression was immunohistochemically detected using the ABC-method.
Appropriate biotinylated secondary antibodies were used for detection using the ABC-method (Vector Laboratories).
Oestrogen receptor alpha (ER), ERBB2 expression was immunohistochemically determined using the ABC-method.
We estimated the parameters under the most likely model using the ABC-GLM method implemented in ABCtoolbox [ 44].
Immunohistochemistry was performed using the ABC-Kit from Dakocytomation.
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