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The proteins were blotted onto a polyvinylidene difluoride membrane using the XCell II blotting module (Invitrogen).
Extracts were fractionated by reducing SDS PAGE on Novex Bis-Tris Mini gels (Invitrogen) and transferred to Invitrolon PVDF membranes (Invitrogen) using the Xcell II blot module.
Proteins were transferred on to nitrocellulose membranes (Bio-Rad) using the Xcell II blot module (Invitrogen).
Proteins were transferred to a nitrocellulose membrane using the XCell II Blot module (Invitrogen).
Proteins were transferred to nitrocellulose membranes (Bio-Rad) using the Xcell II Blot Module (Invitrogen).
Protein samples (20 μg) were separated on 4 12% Bis-Tris gels (Invitrogen) using the XCell II Surelock Mini-Cell system (Invitrogen) and transferred onto polyvinylidene difluoride (PVDF) membranes using the XCell II Mini-Cell Blot Module kit (Invitrogen).
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Proteins were transferred to nitrocellulose membranes using the Xcell blot module (Biorad).
Proteins were transferred to nitrocellulose membranes (Bio-Rad Laboratheies) using the Xcell blot module.
Western blot was performed using the XCell SureLock MINI-Cell (Invitrogen) and the appropriate sample, running, and transfer buffers.
Gel electrophoresis, blotting and protein detection was carried out using the Xcell SureLock Mini-Cell apparatus (Invitrogen) as described previously.
Gel electrophoresis and western blotting were performed using the Xcell Surelock system (Invitrogen) using precast gradient gels (4 20%) as described [68].
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