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The testosterone levels were also measured using the Testosterone ELISA Kit ( i-DNA Biotechnology, Hamburg, Germany).
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We determined testosterone concentrations in serum, IF, and culture medium for duplicate samples using a testosterone ELISA kit (IBL, Hamburg, Germany) following the manufacturer's instructions.
Sera from healthy donors were recruited in cooperation with the University of Lübeck and were investigated using the anti-dsDNA ELISA, antinucleosome ELISA and the Anti-dsDNA-NcX ELISA.
Serum testosterone, FSH, and LH levels were assayed by ELISA using the pathozyme testosterone kit (Omega, UK, OD497) and Eliscan FSH and LH kits [ 13].
ELISA was performed using the Aβ (1-40) Elisa kit II (Wako) and the Aβ (1-42) Elisa kit high-sensitive (Wako) according to manufacturer's instructions.
Cytokine level was determined using the Cytoscreen® ELISA Kit (Biosource).
TNFR1 was measured using the TNFR1 ELISA.
ELISA was performed using the BrdU ELISA kit (Roche Diagnostic Corp., Tokyo, Japan).
Samples positive by ELISA were confirmed by using the inhibition ELISA method (8 ).
Serum testosterone levels were obtained using the ELISA method [ 18].
ELISAs were carried out by using the DS2 Automated ELISA workstation (DYNEX Magellan Biosciences).
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