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Movies were generated directly using the SoftWorx package.
Image deconvolution, projection, and colorization were performed using the SoftWoRx package and Photoshop CC 2014 (Adobe).
Wide-field optical sections at 1-µm z-spacing were acquired with a DeltaVision Elite microscope (Applied Precision) using a 10× N.A. 0.40 air objective, and pseudocolored using the SoftWoRx package or Adobe Photoshop.
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Fixed oocytes (Figures 1 and S1) were imaged on a Deltavision deconvolution microscope using the SoftWoRx software package (Applied Precision).
3D stacks were acquired with z planes separated by 0.2 µm and deconvolved using the Softworx software algorithm (conservative ratio method, 7 iterations).
Raw images were then deconvoluted using the Softworx software and displayed as maximum intensity projections.
Images were collected every 20 45 min for 3 7 h and analyzed using the SoftWoRx software.
All images were deconvolved and maximum intensity was projected using the SoftWoRx software programme (Applied Precision).
The 3D stacks were deconvolved using the Softworx software algorithm (conservative ratio method, 10 iterations; Applied Precision).
Images were processed by constrained iterative deconvolution using the SoftWoRx software (Applied Precision, Issaquah, WA, USA).
Images taken at different focal planes were processed with a deconvolution algorithm and projected into one picture using the Softworx software (Applied Precision).
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