Sentence examples for using the QuikChange protocol from inspiring English sources

The SH3 domain mutations were prepared using the Quikchange protocol (Statagene) and verified by DNA sequencing.

Site-directed mutagenesis was performed using the QuikChange protocol (Stratagene).

Mutants were generated using the QuikChange protocol (Stratagene).

Mutagenesis was performed using the QuikChange protocol, but using KOD polymerase according to manufacturer's protocol.

Site-directed mutagenesis was performed using the QuikChange protocol from Stratagene (La Jolla, CA).

Site-directed mutagenesis was carried out using the QuikChange protocol (Stratagene, La Jolla, CA), following the manufacturers instructions.

A 1.5 kb fragment was subcloned into pCR2.1-TOPO (Invitrogen, Carlsbad, CA), then mutated using the Quikchange® protocol and Pfu Ultra DNA polymerase (Stratagene, La Jolla, CA).

Mutants of Rnd3 (T55A, T55andnd C241S), RhoA (G14V), Syx (E156A/T157A and R178E/K179D) and Syx1b (E164A/R165D) were generated by PCR-mediated musingnesis using the QuikChange II protocol (Stratagene) and sequenced.

The native ELAC2 expression plasmid was modified by site-directed mutagenesis using the QuikChange (Stratagene) protocol: GTCACC replaced (i) the natural 5' untranslated region, or (ii) the 5' untranslated region plus the first 45 nucleotides of coding sequence.

Six codons within the intein sequence were optimized for expression in yeast by site-directed mutagenesis using the QuikChange® protocol (Stratagene, La Jolla, CA): Leu2 (CTC to TTG); Gly5 (GGG to GGA); Leu8 (CTC to TTG); Arg10 (CGA to AGA); Arg46 (CGC to AGA); and Arg49 (CGC to AGA).

45 Introduction of point mutations: Point mutations were introduced using the QuikChange PCR protocol from Stratagene (QuikChange II site-directed mutagenesis kit).