Sentence examples for using the QuikChange approach from inspiring English sources

Point mutations were introduced into the MENTWT gene using the QuikChange approach (Novagen) using oligonucleotides (GeneWorks).

Individual point mutations to alanine were introduced into the pep2 gene of M. smegmatis using the Quikchange approach (Stratagene, primers in Table S1, Supporting Information).

All S. cerevisiae strains and plasmids used are described in Table 3. Plasmid pAC2668 was generated by using the QuikChange Site-Directed Mutagenesis (Stratagene, La Jolla, CA) approach and plasmid pAC2492 (NOT4) as template.

The constructs for mutant of SeADO were generated using the QuikChange site-directed mutagenesis kit (Stratagene).

Saturation mutagenesis libraries were generated using the QuikChange® mutagenesis strategy from Stratagene (USA).

Point mutations in the ZFD of METTL3 were introduced using the QuikChange stratagem, and were verified by DNA sequencing.

Site-directed mutagenesis was performed using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA) and the pPICZ-abf as template.

hDAT pcDNA3.1 was mutated using the QuikChange Mutagenesis kit (Stratagene).

Site directed mutagenesis was performed on pGHLmrA [30] using the QuikChange kit (Stratagene).

Glutamate at amino acid 457 was replaced by glutamine (E457Q) using the QuikChange kit (Stratagene).

Mutations were introduced by PCR-mediated site-directed mutagenesis using the Quikchange kit (Stratagene).