Sentence examples for using the QuickChange protocol from inspiring English sources
Point mutations in the GCP-WD sequence to generate an RNAi resistant version [13] and other mutations were introduced by site-directed mutagenesis using the Quickchange protocol (Stratagene).
For site-directed mutagenesis, LYP1 was inserted into pCR2.1 (Invitrogen) and base substitutions A879T, C885T, G902A, A906G, C927T, C933T, C981A (LYP1-MUTABCDEF) were introduced using the QuickChange protocol (Stratagene), to generate plasmid pKHS318.
Site-directed mutagenesis was performed using the QuickChange protocol.
Point mutants were generated similarly using the QuickChange protocol.
The polymerisation-deficient mutation V339K was introduced using the QuickChange protocol.
Site-directed mutagenesis of p66CH2CB and Prx1 was done using the QuickChange protocol (Stratagene).
Mutations, except DRY/AAY, were generated by PCR with the WT rat AT1a as template using the QuickChange mutagenesis protocol (Stratagene) or as described by Heckman et al., 2007 [32].
The C2098A SNP AChE 3′UTR sequence (in pUC57) was constructed by mutagenesis, using the Quickchange II protocol (Stratagene, CA, USA) (Supplementary Material, Fig. S1G).
All mutants were generated using the Quickchange site-directed mutagenesis protocol.
Mutagenesis was performed using the Quickchange method according to the protocol (Agilent Technologies, UK). Rab proteins in pFAT2 were expressed in BL21 (DE3) pRIL at 18°C for 12 14 hr, then purified using Ni-NTA agarose as described previously (Fuchs et al., 2007).
Amber (TAG) mutations were introduced in pDML924 using the QuickChange PCR site-directed mutagenesis kit and protocol (Stratagene/Agilent Technologies, Santa Clara, CA).
