Sentence examples for using the Primer version from inspiring English sources

The primers were designed using the Primer (version 5.0) software.

All protein-specific primers were designed using the Primer Version 5.0 (PREMIER Biosoft Intern ational) and listed in Table 4.

Real-time PCR analyses were performed using the primer pairs designed using the Primer (version 5.0) software (Additional file 16).

Primers sequences for St genes were designed using the Primer 3 version 0.4.0 http://frodo.wi.mit.edu/primer3/ software (Additional file 7).

Primers that were specific for the NA gene of N9 influenza viruses were designed using the Primer Explorer version 4 software (Eiken Chemical Co., Ltd., Tokyo, Japan; http://primerexplorer.jp/elamp4.0.0/index.html) and synthesized by Shanghai Invitrogen Co., Ltd.

More than 30 primers for amplions of PPO genes were designed using the Primer 3 (version 0.4.0) [ 40] based on available gene specific nucleotide sequence information retrieved from the GenBank databases.

To generate VIGS constructs, PCR fragments ranging from 246 to 253 bp in size were amplified from the sorghum candidate gene using genomic DNA of the resistant GA06/18 genotype and gene-specific primers harboring NcoI and AvrII restriction sites using the Primer 3 version 0.4.0 http://frodo.wi.mit.edu/primer3/ software (Additional file 6).

Primers were designed using the Primer Premier program (version 5.0).

The gene-specific primers were designed based on the sequencing results using the Primer Premier software (version 6.0).

Specific primer pairs of thirteen candidate unigenes with potential roles in three secondary metabolic pathways designed for real time RT-qPCR using the Primer premier software (version 5.0) were shown.

Statistical analysis was performed using the Primer of Biostatistics system, version 5.0 [38].