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The protein domains of identified TF were classified using the Pfam database [122].
The metagenomic libraries were also analyzed using the Pfam database as a comparison to the SEED platform conclusions.
After CTT and sequence similarity-based annotations combined with HMMER3 predictions (E-value <1) using the PFAM database [28] (Version 24.0, released in October 2009, 11,912 protein families), we discovered a collection of 3,177 new FBX loci.
The domain annotations were assigned using the Pfam database.
Protein domains were then predicted using the Pfam database.
Domain information was assigned using the Pfam database with a threshold E-value of 1.0 [ 30].
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To define protein families, we used the Pfam database which contains protein family models based on Hidden Markov Models [44], [57].
For training of the Multiple Pfam family based FDR recognition component of EFICAz, we use the Pfam database [ 83].
We used the Pfam database version 24.0 to assign protein domains to the vacuolar pyrophosphatase sequences [ 42].
Subsequently, we used the Pfam database [ 22] to find domains in the 43 proteins in order to distinguish proteins whose multi-functionality originates from different domains.
We then used the Pfam database to identify the events of gain or loss of different protein families between these two species.
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