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Western blot scanning and analysis was conducted using the Odyssey system and its associated software (LiCor).
Proteins were visualized by chemiluminescence using Supersignal (Pierce) or by fluorescence using the Odyssey system (Li-Cor Biosciences, Lincoln, NA).
The immunoblotting conditions were the same as described above and western blot scanning and analysis was conducted using the Odyssey system and its associated software (LiCor).
Detection and relative quantification were done using the Odyssey system (V3.0, LI-COR Biosciences).
The blots were detected using the Odyssey system (Li-COR, Lincoln, NE, USA).
The membrane was washed again and scanned using the Odyssey system (LI-COR Biosciences, USA).
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Four-micrometre-thick sections were scanned using the Odyssey imaging system at the highest (21 μm) resolution and quality.
Western blots were analysed using HRP-conjugated secondary antisera (Sigma), or using the Odyssey infrared system (Licor, Lincoln, NE, USA).
Blots were imaged using the Odyssey Fc system (LI-COR, Lincoln, NE, USA) and analysed using Image studio v2.0 software.
Proteins were visualized using the Odyssey imaging system (LI-COR Biosciences, Cambridge, UK), or enhanced chemiluminescence (for caspase-8).
Immunoreactive bands were detected using the Odyssey imaging system (LI-COR Biosciences) and quantified using ImageQuant software (Amersham Biosciences, Piscataway, NJ, USA).
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