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Cardiovascular autonomic function tests were measured using the O'Brien protocol.
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The oral quinine group met the predefined stopping rule of a significant difference in cure rate using the O'Brien Fleming method, with P<0.001, and the study was halted.
Using the O'Brien-Fleming criteria for two-sided test, the alpha-level of this interim analysis was 0.005.
The nominal one-sided significance level for the interim analysis determined by using the O'Brien-Fleming stopping rule is 0.0015.
Using the O'Brien-Fleming method (O'Brien and Fleming 1979) results in respectively |4.495|, |3.178| and |2.595| as critical values for the Z-statistic at the three analysis time points.
Using the O'Brien-Fleming criteria for two-sided test, the alpha-level of this interim analysis will be 0.005.
Using the O'Brien-Fleming method [ 12] in the interim analysis, the significance level in the final analysis is estimated to be 4.9% for a two-sided test.
The difference between the two groups in the period up to the event occurrence, using the O'Brien-Fleming alpha-spending function, will be examined for statistical significance.
All analyses will be adjusted for the fact that an interim analysis will be performed using the O'Brien-Flemming alpha spending function [ 18].
We use the O'Brien-Fleming Method to calculate the stopping boundary.
30 O'Brien et al, 31 using the British Hypertension Society protocol, graded the Dinamap 8100 B for systolic blood pressure and D for diastolic blood pressure compared with the conventional mercury sphygmomanometer in adults.
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