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Tumors were graded using the Nottingham system [ 40– 40].
Grade was determined using the Nottingham System and classified as grade 1, 2 or 3 (Elston and Ellis, 1991).
The final pathological tumor stage was determined using the TNM staging system (AJCC Cancer Staging Manual, 7th edition, 2007) and graded using the Nottingham system [ 13].
Tumour grade and necrosis was scored on haematoxylin- and eosin-stained sections immediately adjacent to those sections subjected to immunohistochemistry. Tumour grade was assessed using the Nottingham system (Elston and Ellis, 1991).
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Tumor grade was determined using the Nottingham grading system [ 26].
The histological grade was assessed using the Nottingham grading system [ 13].
These tumors spanned a wide range of grades (grades 5-9, median 7.5), which were determined using the Nottingham grading system [ 23].
A hematoxylin and eosin (H&E) slide for each case was reviewed to confirm tumor grade by using the Nottingham grading system to include tubules, nuclear grade, and mitosis.
The histologic grade of all cancer slides was determined using the Nottingham histologic grading system [ 19].
Histological grading is an important part of breast tumour classification, and is performed using the Nottingham histological grading system.
Tumors were classified according to the WHO histologic classification of breast tumors and graded using the Nottingham histologic grading system [ 40, 41].
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