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Microarray was carried out using the NimbleGen microarray service.
Array data (See Additional file 18) were normalized using the NimbleGen microarray data processing pipeline (NMPP) [ 65].
In a genome-wide expression analysis using the NimbleGen microarray platform, of Auxin/Indole-3-Acetic Acid (Aux/IAA) and Auxin Response Factor (ARF) in Populus (Populus trichocarpa clone Nisqually-1), the genes in a subgroup of Aux/IAA showed differential expression among different tissue types [ 18].
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To test whether the association of MYCN at sites of hypermethylation is correlated with reduced expression of genes, we have analyzed the Kelly and SK-N-AS cell lines using the NimbleGen expression microarray platform.
We verified these predictions by performing a ChAP-chip experiment using the Nimblegen promoter microarray.
Oligonucleotide array comparative genomic hybridization was performed using the Nimblegen 135 K microarray (Roche Nimblegen) or Agilent 44 K/60 K/75 K/105 K microarrays (Agilent Technologies) in an accredited clinical laboratory in accordance with manufacturer's instructions.
The arrays were dried for one minute using a High-Speed Microarray Centrifuge (Arrayit Corp., Sunnyvale, Ca).. Image acquisition was attained using the NimbleGen MS 200 Microarray Scanner (Roche NimbleGen, Inc). at 2 μm resolution.
Images were acquired by using The NimbleGen MS 200 Microarray Scanner (NimbleGen Systems, Inc., Madison, WI) at a 5 μm resolution.
Six biological replicates, comprising two clusters each, were separated into skins and pulp in preparation for RNA extraction and transcriptomic analysis using the NimbleGen Grape Whole-Genome Microarray.
Methylated DNA immunoprecipitation followed by microarray analysis using the Nimblegen '3 × 720 K CpG Island Plus RefSeq Promoter' platform was applied in order to carry out genome-wide DNA methylation profiling covering 20 404 promoter regions.
Following hybridization, the microarray was washed using the NimbleGen Wash Buffer Kit.
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