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The complete RNA polymerase sequences were analyzed by the neighbor-joining method using the NEIGHBOR program in Phylogeny Inference Package (PHYLIP) [ 29].
The best alignments of individual genes were analyzed by a neighbor-joining method using the NEIGHBOR program in Phylogeny Inference Package (PHYLIP) [ 35].
The trees depicted in Figures 5 through 8 are neighbor-joining trees calculated using the NEIGHBOR program from PHYLIP version 3.6a2.1 [ 30].
Neighbor-Joining trees were calculated using the NEIGHBOR program from the PHYLIP package (Felsenstein 1993).
Neighbor-joining (NJ) trees were constructed using the NEIGHBOR program in PHYLIP and were drawn with TreeView.
Neighbor-Joining Distance (NJD) trees were generated using the NEIGHBOR program and consensus trees were generated with CONSENSE.
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Branch lengths for each structure alignment from MAMMOTH-mult (25) were measured by our in house software and minimized using the neighbor joining program implemented in Phylip (24).
We then used each similarity matrix to generate corresponding dendrograms using the neighbor-joining program in PHYLIP [44] and visualized with the Phylodendron phylogenetic tree printing webserver (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html, accessed: 11/09/2009).
A dendrogram was generated from the similarity matrix using the neighbor-joining program that is part of the PHYLIP package (http://evolution.genetics.washington.edu/phylip.html).edu/phylip.html
To examine the evolutionary relationships among the WRKY domains, we estimated the phylogeny by using the neighbor-joining program from PHYLIP 3.57 for the amino acid sequences of WRKY domains from G. lamblia, the slime mold, the green alga, Arabidopsis and rice.
We constructed a greedy-consensus (Bryant 2003) neighbor-joining tree (Saitou and Nei 1987) using the neighbor and consensus programs in the phylip package (Felsenstein 2008) from 1000 bootstrap resamples across loci, and we visualized the tree with Dendroscope (version 3) (Huson and Scornavacca 2012).
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