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For the relative gene expression in rice and nematode samples, data were analyzed using the MxPro software package to obtain the relative expression levels of rice genes.
Data were analyzed using the MxPro software.
Data were analyzed using the MxPro software package.
The results were automatically determined using the MxPro software (Stratagene).
The samples were analyzed and normalized against HPRT-1 using the MxPro software (Stratagene).
Ct values were determined using the MxPro software (Stratagene, La Jolla, CA).
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Data analysis was performed by using the MxPro qPCR software package (Stratagene, La Jolla CA).
The threshold cycle (Ct) value for each amplified sample and each gene of interest was measured by using the MxPro QPCR software (Stratagene), and values were normalized to GAPDH expression by using the 2-ΔΔCt method (Frisch et al. 2016).
The threshold cycle (Ct) value for each gene of interest was measured for each amplified sample using the MxPro QPCR software (Stratagene) and values were normalized to GAPDH expression using the 2-ΔΔCt method as previously employed (Cucchiarini et al. 2011; Frisch et al. 2015a; Venkatesan et al. 2012).
Analysis was performed using the MxPro 4.01 software.
Quantitative PCR (FastStart Universal SYBR Green Master Rochee) reactions were run on an MX3005P thermocycler (Stratagene, La Jolla, CA, USA) and analysed using the MxPro QPCR software, version 4.01 (Stratagene, Agilent Technologies, Santa Clara, CA, USA).
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