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Plasmid DNA was isolated using the Miniprep kit (QIAGENGmbH, Hilden, Germany).
The plasmids were introduced into S. aureus strain RN4220 by electroporation and purified using the miniprep kit from Omega.
The product extracted using the Miniprep kit (Qiagen) was then sequenced (3130XL Genetic Analyzer, Applied Biosystems).
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The remaining re-suspended culture was used to perform triplicate 500µL minipreps using the Zyppy miniprep kit (Zymo Research).
Plasmids were purified from E. coli using the Zyppy miniprep kit (Zymo Research) or the GenElute mimiprep kit (Sigma-Aldrich).
The recombinant plasmid pHR2 was isolated from L. reuteri HR2 using the plasmid miniprep kit, with the following modifications: The cells in resuspension buffer, were lysed with 30 mg/mL lysozyme (USB) and incubated at 37°C for 30 minutes.
Total RNA was isolated using the RNeasy Miniprep kit (Qiagen).
The reconstructed plasmids were purified from the transformed XL10-Gold using the QIAprep Miniprep kit.
Plasmid DNA was isolated from individual clones using the QIAprep miniprep kit (Qiagen).
For RT-PCR analysis, total RNA was first extracted from retrovirally-infected cells using the RNAeasy Miniprep Kit (Qiagen).
Total RNA was extracted from infected NIH3T3 cells using the RNAeasy Miniprep Kit (Qiagen; Valencia, CA) according to the manufacturer's instructions.
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