Sentence examples for using the MessageAmp™ ii from inspiring English sources

400 ng of total RNA was amplified using the "MessageAmp™ II aRNA Amplification Kit" from Ambion.

mRNA amplification was performed using the MessageAmp™ II Biotin Enhanced kit from Ambion, according to the manufacturer's instructions.

For the microarray hybridization, the RNA from the samples and the reference pool (1μg of each sample) were amplified and aminoallyl-labeled using the MessageAmp™ II aRNA Kit (Ambion, http://www.ambion.com) and 5- 3-aminoallyl -20-deoxyuridine-50-triphosphate (aa-dUTP, Ambion), following the manufacturer's instructions.

Fragmented and biotin-dUTP-labeled amplified mRNA was generated from purified staphylococcal RNA using the MessageAmp™ II-Bacteria Kit (Ambion) according to the manufacturer's protocol.

RNA from unstimulated DC was included as a negative control. 1 µg RNA was converted into cDNA, and biotin-labeled aRNA was synthesized using the MessageAmp™ II-Biotin Enhanced Kit (Ambion, Austin, TX, USA) according to the manufacturer's instructions.

We used the MessageAmp™ II aRNA Amplification kit (Ambion, Austin, TX, USA) that preferentially amplifies eukaryotic mRNA.

For microarray assay of miRNAs, double-stranded cDNAs were synthesized with the 2 μg total RNA, and biotin-tagged cRNAs were obtained by the use of the MessageAmp™ II aRNA Amplification Kit (Ambion).

After extraction, RNA was amplified by using the MessageAmp II aRNA Amplification Kit (Ambion).

Both sample and reference RNAs were amplified using the MessageAmp II aRNA amplification kit (Ambion, Austin, TX).

Total RNA (2 3 ng) was amplified using the MessageAmp II aRNA Amplification Kit (Ambion, USA) according to manufacturer's recommendations.

RT, second strand synthesis, cDNA purification and in vitro transcription with Biotin-11-UTP were performed using the MessageAmp II aRNA Amplification Kit (Ambion).