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Genotyping was carried out using the MassARRAY platform [33].
DNA methylation at individual CpG units was quantified using the MassARRAY platform, as previously described [ 22].
DNA methylation levels at individual CpG sites were evaluated quantitatively using the MassARRAY platform (Sequenom, San Diego, CA).
Genotyping of SNPs was performed using the MassARRAY platform from Sequenom (San Diego, USA) at the VIB Vesalius Research Center, Leuven, Belgium.
We subsequently amplified five amplicons (A-1 to A-5) by PCR, which were subjected to quantitative DNA methylation analysis using the MassARRAY platform as previously described [ 22].
Concordance of genotype calls between the different platforms was confirmed by genotyping 87 control DNAs for all 8 markers using the MassARRAY platform with strong concordance of calls between technologies 98.99% for the 8 markers overall.
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SNP genotyping was performed using the MassARRAY iPLEX platform.
DNA methylation levels at the HBG2 promoter were measured using the MassArray EpiTYPER platform (Sequenom Inc., CA, USA), which quantitatively analyze fragmented PCR products using MALDI-TOF mass spectrometry.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was performed using the MassARRAY EpiTYPER assay platform (SEQUENOM Inc., San Diego, CA, USA) on bisulfite-converted DNA as previously described [ 37].
Genotyping was performed using the MassARRAY iPLEX Gold™ platform (Sequenom, San Diego, CA USA) and TaqMan™ allelic discrimination assays (Applied Biosystems, Foster City, CA, USA).
Primers were designed using the MassARRAY Assay Design software.
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