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Processed files were searched against the SwissProt human database using the Mascot search engine version 2.3.0.
The PKL files were analyzed using the MASCOT search engine.
Digested proteins were further analyzed by MALDI-TOF MS. Mass spectra values were searched against NCBI and Swiss-Prot protein databases using the Mascot search program (http://www.matrixscience.com).com
Peptide matching and protein searches against the NCBI nonredundant (nr) databases were performed using the Mascot search engine (http://www.matrixscience.com) with a mass tolerance of ±0.3 Da.
The data were analyzed using the MASCOT search program (Matrix Science, London, UK).
Peak lists obtained from the mass spectra were used to identify proteins using the Mascot search engine (Matrixscience).
The peptides were searched using the Mascot search engine (Matrix Science) against the human protein database NCBInr.
The obtained spectra were finally analyzed using the DATA EXPLORER program and proteins were identified using the MASCOT SEARCH website.
The database search was performed by using the MASCOT search engine (Matrix Science, London, United Kingdom) to screen the NCBI protein sequence database restricted to rat taxonomy.
The MS/MS fragmentation data achieved was used to search the National Center for Biotechnology Information and Flybase databases using the MASCOT search engine (http://www.matrixscience.com).com
The MS data were analyzed using MaxQuant [47] and proteins identified by searching MS and MS/MS data using the MASCOT search engine (Matrix Science, UK).
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