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The results were visualized using the MapMan tool.
We using the MapMan tool view annotated processes of interest or metabolic pathways by groups of rubber tree transcripts.
Additionally, by using the MapMan tool, it was found that changes in expression of several genes related to distinct regulation pathways were quite similar in the DnaJ mutants at GL conditions to those recorded in the HL-treated WT (Additional file 5).
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To analyze the relationship of DE genes with metabolic processes, we used the MapMan tool [ 25] to visualize the distribution of DE genes on known metabolic pathways.
As a first step, we used the MapMan tool [ 30] for displaying the transcriptional differences between T- and S-oaks after 16 h of T. viridana feeding to obtain a global overview of the related cellular pathways.
Functional analysis of differentially expressed genes was performed using the MapMan software tool [ 69] using ontology adapted for potato [ 70].
Using the MapMan visualization tool, relevant metabolic routes can be analysed in greater detail including secondary metabolites, glycolysis, transport genes or transcription factors, providing insight into tissue dependent differential regulation and whether the underlying genetic component is either cis- or trans-acting.
The cluster was significantly enriched for genes with known or putative receptor kinase function and genes implicated in calcium signalling when compared to all the probesets on the array using the Mapman over-representation tool (http://mapman.mpimp-golm.mpg.de/general/ora/ora.shtml, [ 37]).
In addition, the differentially expressed genes were also functionally analyzed using the MapMan software, which is a user-driven tool that displays large genomics datasets onto diagrams of metabolic pathways or other processes [ 54].
Gene function enrichment analysis was performed using the Mapman categories through the sequence annotation file obtained using the Mercator web tool [ 38].
The mapping files for the Mapman representation were made using the Mercator web server [ 38], and the Mapman images were made using the Mapman software [ 66].
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