Sentence examples for using the ImProm ii from inspiring English sources

cDNA was prepared from 50 ng of RNA using the ImProm II Reverse Transcription System (Promega).

Reverse transcription was carried out using the ImProm II reverse transcriptase (Promega, Madison, WI).

cDNA was synthesized from 1 ug of RNA using the ImProm II Reverse Transcriptase kit and random hexamer primers.

First strand cDNA synthesis was performed using the Improm II reverse transcriptase enzyme (Promega, Wisconcin, USA) according to the manufacturers' protocol.

Total RNAs were isolated from cells or tissues using RNAiso Plus (Takara, Shiga, Japan), and cDNAs were made using the Improm II Reverse Transcription System (Promega, Madison, WI, USA).

One microgram of total RNA from each tissue was used for oligo-dT primed cDNA synthesis, which was performed using the ImProm II Reverse Transcription System (Promega) following the manufacturer's instructions.

According to manufacturer's instructions, first strand cDNA synthesis was carried out using the ImProm-II™ Reverse transcription system (Promega, Madison, USA).

Briefly, 3 µg of total RNA were used for first and subsequent second strand cDNA synthesis using the ImProm-II reverse transcription system (Promega, Madison, WI).

Total RNA (1 µg) was reverse-transcribed to cDNA using the ImProm-II RT kit (Promega, Madison, WI) with random hexamer primers according to the manufacturer's instructions.

One µm of RNA was used for reverse transcriptase polymerase chain reaction (RT-PCR) in a total volume of 20 µl with oligo dT 15 primer using the ImProm-II reverse transcription system (Promega) according to the manufacturer's instructions.

Total RNA samples from day8 wild type and VRK1-deficient mice (n = 4, each) were prepared from intact testicular cells using Trizol (Invitrogen, Carlsbad, CA) and then reverse transcribed using the ImProm-II Reverse Transcription System (Promega).