Sentence examples for using the IPGphor system from inspiring English sources

Isoelectric focusing was performed using the IPGphor system (Amersham Pharmacia).

PIEF was performed using the IPGPhor system and 24-cm IPG strips, pH 3-10 (GE Healthcare, Piscataway, NJ).

A total of 200 μg of proteins were applied on immobilised linear gradient strips (pH 4 7) using the IPGphor system (Amersham Pharmacia Biotech, Uppsala, Sweden).

First-dimension isoelectric focusing was carried out using the IPGphor IEF system (GE Healthcare) for 9 to 11 h (a total of 45 to 60 kVh).

Samples were applied to 24-cm, pH 3.0 10.0 IPG strips (Bio-Rad), and isoelectric focusing was performed using the IPGphor IEF system (GE Healthcare).

Proteins extracts (30 µg) from epididymal fat pads were loaded onto an Immobiline Drystrip IPG gel (Amersham Biosciences, pH range 3 10 NL), and proteins were separated using the IPGphor Isoelectric Focusing System (Amersham Biosciences) according to manufacturer's instructions.

Gel rehydration was carried out for 14 h at 50 V and focused using the IPGPhor (Amersham) isoelectric focusing apparatus.

Isoelectric focussing was performed using the IPGphor (GE Healthcare) for a total of 50 kV/hrs at 20°C with the resistance set at 50 mA per strip.

Proteins were first separated according to their isoelectric point along linear immobilized pH-gradient (IPG) strips (pH 3-10, 18 cm long) using the IPGphor apparatus (Amersham Biosciences).

IEF was conducted using the Ettan IPGphor system (GE Healthcare), as previously described (Weiss et al. 2009).

Iso-electric focussing (IEF) was performed for a total of 95,000 Vh for 17 cm strips and 70,000 Vh for 11 cm strips at 20°C using the IPGphor-II apparatus (GE Healthcare, Hercules, UK).