Following amplification and product purification, molecular masses and base counts of amplicons were determined, using the T5000 platform, as previously described in detail [25], [42] [45].
Libraries were prepared and sequenced using the HiSeq2500 platform at the UC Davis DNA Technologies Core Facility (http://dnatech.genomecenter.ucdavis.edu).edu
PCR was performed using the Mx3000P platform (Agilent) using the following conditions: 45 cycles of 95°C for 12 seconds and 60°C for 35 seconds.
Paired-end libraries were pooled according to the attached adaptors and sequence analysed using the HiSeq2000 platform (Illumina) following the standard manufacturer's protocol.
We generated 138.7 M reads with an average of 11.6 M reads per library from the samples sequenced using the HiSeq2500 platform.
The whole exome was characterized by using the HiSeq2000 platform (Illumina, San Diego, CA) and SureSelectXT Human All Exon V4 for exon enrichment (Agilent, Santa Clara, CA).
For early-stage cancer, no target gene is reported by using the GSE7670 platform; therefore, we report common drug target genes among the rest of the other three microarray platforms.
For example, sequencing 200 human individuals with the genome size of ∼3 Gb would require 5.2 billion reads, which are approximately equivalent to the number of reads produced from >4 full sequencing runs using the HiSeq2000 platform.
Much like Mr. Clinton in 1992, Gov. George W. Bush of Texas has tried to use the 2000 platform to capture a broader swath of moderate swing voters.
Capillary sequencing of these amplicons used the 3730xl platform (Applied Biosystems, CA, USA).
