Three-color flow cytometry data were acquired using CELLQuest software (Becton Dickinson) and then analysed using the FlowJo software package (Tree Star Inc .. Data are representative of three independent experiments of 9 × 10cells per sample with ⩾50 000 events collected.
The FACS profiles were converted to line graph data using the FlowJo analysis software (Treestar, Ashland, OR, USA).
Data analyzed using the FlowJo software v7.6.1.
Ten thousand cells were examined on a FACSCalibur flow cytometer (Beckton Dickinson, Franklin Lakes, NJ) and data analyzed using the FlowJo software (Tree Star, Ashland, OR).
Counting of mCherry+eGFP− and mCherry+eGFP+ cells was performed on LSRFortessa (BD Bioscience, France), and data analyzed using the Flowjo software (Tree start, Ashland, Or, USA).
After 40 min incubation at 37 °C, samples were accessed with the Fortessa flow cytometer (BectonDickonson) and data analysed using the FlowJo software (TreeStar).
Data was analyzed using the FlowJo software (TreeStar) version 8.8.6.
Data was analyzed using the FlowJo software (TreeStar Inc., Ashland, OR).
The data were analyzed using the FlowJo software program (Tree Star, Ashland, OR).
Data were analyzed using the Flowjo software (Tree Star, Inc., Ashland, Oregon, http://www.treestar.com).
