Sentence examples for using the Fixed modification from inspiring English sources
The searches were run using the Fixed modification of iodoacetamide labelled cysteine parameter enabled.
Carbamidomethylation of cysteine was used as the fixed modification and oxidation of methionine as variable modifications.
Carbamidomethylation of cysteine was used as the fixed modification and oxidation of methionine and protein N-terminal acetylation as variable modifications.
The fixed modification carbamidomethyl (C) +57.02146 Da was applied.
Carbamidomethylation was set as the fixed modification and oxidation of methionine was set as the variable modification.
Search parameters used the fixed cysteine carbamidomethylation and the variable methionine oxidation as modifications, and matches were judged by the number of peptide sequence tags, sequence coverage, MOWSE score, and the quality of tandem MS spectra (See Additional File 1).
The fixed predictor uses the fixed coefficients.
Database searches were performed with MASCOT (www.matrixscience.com) using the following settings: fixed modifications, carbamidomethyl (C); variable modifications, oxidation (M), peptide charge 2+ and 3+.
Enzyme specificity was set to trypsin with one missed cleavage using carbamidomethylcysteine as fixed modification and phosphorylation of S/T/Y as variable modification.
Raw data were converted into MGF format and database searches were performed on a MASCOT server (MASCOT 2.2, MS/MS Ion Search) using fixed modification (none), variable modification carbamidomethyl (Cys), and methionine oxidation for each protein band sample (16 digested samples).
Carbamidomethylation of cysteine was used as fixed modification whereas oxidation of methionine and protein N-terminal acetylation were used as variable modifications.
