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cDNA probes were synthesized using the FairPlay microarray labeling kit (Stratagene, La Jolla, CA).
For each microarray experiment, 20 μg of total RNA were reverse transcripted and indirectly labelled using the FairPlay Microarray Labelling Kit (Stratagene).
Twenty micrograms of RNA were used for cDNA generation, and cDNA labeled with Cy3 or Cy5 monofunctional reactive dye to amino allyl-modified dUTP incorporated into cDNA using the FairPlay Microarray labeling kit (Stratagene).
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Total RNA (5 µg) was reverse transcribed using the Fairplay III Microarray Labeling kit (Stratagene) and labeled with Cy3-dCTP or Cy5-dCTP.
Fluorescently labelled cDNA was generated from 20 μg total RNA using the FairPlay II microarray labelling kit (Stratagene), following the manufacturer's protocol.
Briefly, cDNA was generated from 12.5 μg of total RNA and labeled with either Cy3 or Cy5 fluorescent dyes using the FairPlay III Microarray Labeling System (Stratagene).
Amino allyl modified cDNA was generated by reverse transcription from 20 μg of total RNA and labeled with either Cy3 or Cy5 mono-reactive fluorescent dyes using the FairPlay III Microarray Labeling System (Stratagene).
Ten micrograms of total RNA was reverse transcribed using the Fairplay III Microarray labeling kit (Agilent Technologies) and cDNA were indirectly labeled using Cy3 or Cy5 Mono reactive dyes (GE Healthcare).
RNA was reverse transcribed and labelled with either Cy3 (pooled reference) or Cy5 (FO, RO, SO and LO fed fish) fluors using the FAIRPLAY II cDNA indirect labelling kit (Stratagene) according to the manufacturer's instructions.
Cy3 or Cy5 was incorporated into each sample using the Fairplay labelling kit (Stratagene, La Jolla, CA) and the labelled cDNA cleaned-up after passage through DyeEx columns (Qiagen Ltd., Crawley, UK).
Total RNA was reverse transcribed and labelled with either Cy3 or Cy5 using the Agilent Fairplay Microarray Labelling kit (Stratagene).
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