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Sodium bisulfite treatment was carried out using the CpGenome DNA modification kit (Chemicon).
Bisulfite treatment of DNA was achieved using the CpGenome DNA Modification Kit (Chemicon) according to the manufacturer's instructions.
Genomic DNA was subjected to bisulphate conversion using the CpGenome DNA Modification Kit (Intergen) according to the manufacturer's protocol.
Briefly, 0.5 1.0 µg of genomic DNA was treated using the CpGenome DNA modification kit (Chemicon), including DNA sulfonation, deamination, desalting, desulfonation and recovery.
Then, DNA (1 μg) was subjected to bisulfite modification using the CpGenome DNA Modification Kit (Millipore, Billerica, MA).
Genomic DNA from 11 ACLs was bisulphite modificated using the CpGenome DNA modification kit (CHEMICOM International, Inc., Temecula, CA, USA) following the manufacturer's instruction.
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Sodium bisulphite modification of genomic DNA was performed using the CpGenome Universal DNA Modification Kit (Chemicon International, Temecula, CA, USA).
The bisulfite modification of genomic DNA was performed using the CpGenome Turbo Bisulfite Modification Kit (Billerica, MA, USA), and the methylation analysis of LINE-1 repetitive elements was performed initially using the COBRA assay.
Genomic DNA was treated with sulfite using the CpGenome Turbo Bisulfite Modification Kit (Millipore, CA, USA) according to the manufacturer's instructions.
In total, 1 μg of DNA was treated with sodium bisulphite for 16 h, using the CpGenome kit (Serologicals, UK), according to the manufacturer's instructions.
Bisulfite treatment was performed using the CpGenome modification kit (Chemicon) according to the manufacturer's recommendations.
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