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Coiled-coil regions were predicted using the Coils2 server (http://www.ch.embnet.org).org
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Plus, the company claims that using the K3 Server system instead of traditional processes results in a 50% reduction in deployment, operation and maintenance of enterprise integrations.
The CsACS2 three-dimensional structures were generated using the Geno3D server (http://geno3d-pbil.ibcp.fr).ibcp.fr
Natively unstructured/disordered region was predicted using the DISOPRED2 server [79], which is one of the leading servers for predicting natively disordered regions in proteins.
The open reading frame (ORF) of the PNO of Grammia geneura was analyzed for the presence of an N-terminal signal peptide by using the SignalP3.0 server [39].
Transmembrane helices were predicted using the TMHMM2.0 server [ 35, 36].
Sequence-structure threading was performed using the 3DPSSM server.
Finally, primer sequences were designed using the PRIMER3 server [ 40].
An atomic resolution model of PfaE3 was generated using the PHYRE2 server [ 31].
Secondary structures for the Ago2 sequence were predicted using the JPRED3 server[ 25].
ESTs representing the same locus were assembled using the CAP3 server at Iowa State University [ 61].
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