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Data were analyzed using the CellQuestTM software.
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Analysis was performed using the software application CELLQuestTM (Becton Dickinson).
Two additional washes were performed and cell surface antigen expression was analyzed on a FACScan using CellQuestTM software (both BD Biosciences, San Jose, CA, USA) with 10,000 events recorded for each sample.
In all cases, microparticles were incubated with the antibodies or relevant isotype controls for 45 min at 4°C prior to analysis with a FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA, USA) using CellQuestTM software (Becton Dickinson).
The automaker has already admitted using the deceptive software.
Simulations were performed using the Matlab/Simulink software.
Simulations are performed by using the Matlab/Simulink software.
Cells were counted using the ImageJ software.
Data were analyzed using the instrument software.
Analysis was done using the manufacturer software.
Data were graphed using the Prism software.
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