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Tibial lead was evaluated in subjects with different VDR genotypes defined using the BsmI restriction enzyme, adjusting for confounding variables.
To genotype the samples for the BsmI polymorphism (rs1544410), a RFLP analysis was carried out by using the BsmI restriction endonuclease enzyme (R0134S New England BioLabs).
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Within the amplified PCR product, the BsmI restriction site is present only in the coi1-1 mutant.
Fragments of 650 and 150 bp are visible if the 800 bp product is cut by the BsmI restriction enzyme.
The presence of the BsmI restriction site generated 175 bp and 650 bp fragments, whereas the absence of this site yielded an 825 bp fragment.
AY342401] consists of the common allele, denoted b, and a variant, denoted B, in which the BsmI restriction enzyme site is absent.
A total of 169 (33.5%) subjects were homozygous for the BsmI restriction site (designated bb), 251 (49.8%) were heterozygous (Bb), and 84 (16.7%) were homozygous for the absence of the restriction site (BB).
Dendrograms were constructed using the restriction patterns generated by different restriction enzymes for 12 framework species.
pCC1FOS-96 DNA was partially digested using the restriction enzyme Sau3A1.
Restriction digestion was performed using the EpiTect Methyl II DNA Restriction Kit (SAB# 335452).
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