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Electroblotting was done using the BioRad Criterion blotter.
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The protein concentration was measured using the Biorad Protein Assay and BSA as a standard.
The effect of the presence of HbS in the determination of HbA2 using the Biorad Variant II analyzer.
Semi-quantitation of the protein bands was performed using the Biorad Chemi doc MP imaging system.
Q-PCR was performed on a BioRad icycler using the BioRad SYBR green master mix.
Samples were quantified for protein concentration using the BioRad Protein Assay Kit (BioRad, Canada).
Quantitative PCR reactions were performed using the BioRad Chromo4 detection system (BioRad, Mississauga, ON).
The images were reconstructed using the BioRad LaserSharp software.
The protein concentration was determined using the Biorad protein assay.
PCR was performed using the Biorad iCycler (Hercules, CA).
Protein samples were quantified using the BioRad Protein assay.
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